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We have shown that pluripotent stem cells can be generated
from mouse embryonic fibroblasts (MEF) and adult mouse tail tip
fibroblasts by the retrovirus-mediated transfection of four transcription
factors, namely Oct3/4, Sox2, c-Myc, and Klf4. We designated these cells
as iPS (induced pluripotent stem) cells. Mouse iPS cells are indistinguishable
from ES cells in morphology, proliferation, gene expression and teratoma
formation. Furthermore, when transplanted into blastocysts, mouse iPS cells
derived from MEF can give rise to adult chimeras, which are competent for germline transmission.
These results are proof-of-principle that pluripotent stem cells can be
generated from somatic cells by the combination of a small number of factors.
Selected Publications
Takahashi K., and Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126: 663-676, 2006
Takahashi K., Maruyama M., Tokuzawa Y, Murakami M, Oda Y, Yoshikane N, Makabe KW, Ichisaka T,
Yamanaka S. Evolutionarily Conserved Non-AUG Translation Initiation in NAT1/p97/DAP5 (EIF4G2), Genomics 85:360-71, 2005
Nakamura, Y., Miura, K., Fujino, Y., Iwao, H., Ogita S. and
Yamanaka S. Evolution, Structure, and Expression of GNPI/Oscillin Orthologous Genes. Genomics 68: 179-186, 2000
Information last updated September 2007
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